Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 28 publications below |
| IHC | See 11 publications below |
| IF | See 12 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
| ChIP | See 3 publications below |
Product Information
22242-1-AP targets REST in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17707 Product name: Recombinant human REST protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 748-1097 aa of BC132859 Sequence: ELSPPMEVVQKEPVKIELSPPIEVVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEVGLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEALAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE 相同性解析による交差性が予測される生物種 |
| Full Name | RE1-silencing transcription factor |
| Calculated molecular weight | 1097 aa, 122 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | BC132859 |
| Gene Symbol | REST |
| Gene ID (NCBI) | 5978 |
| RRID | AB_2879044 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13127 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for REST antibody 22242-1-AP | Download protocol |
| IP protocol for REST antibody 22242-1-AP | Download protocol |
| WB protocol for REST antibody 22242-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Hepatocyte BDNF Acts as a Novel Immune Checkpoint to Restrain TLR4-Mediated Acute Hepatitis. | ||
Sci Adv YAP drives fate conversion and chemoresistance of small cell lung cancer.
| ||
Sci Adv EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency | ||
Cell Death Differ The cross talk of two family members of β-TrCP in the regulation of cell autophagy and growth. | ||
Mol Biomed Prolactin-Releasing Hormone Receptor (PRLHR) enhances radiosensitivity and exacerbates DNA damage in glioblastoma post-irradiation by inhibiting Y-box-binding protein-1 (YBX1) nuclear translocation: a novel perspective on precision radiotherapy. | ||
Cell Death Dis Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury. |