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  • Featured Product
  • KD/KO Validated

PSAP Monoclonal antibody

PSAP Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11B2

Cat no : 66473-1-Ig

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

Validation Data Gallery

Applications:
  • WB analysis of human milk using 66473-1-Ig

    WB analysis of human milk using 66473-1-Ig

    human milk were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66473-1-Ig

    WB analysis of HeLa using 66473-1-Ig

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells.

  • WB analysis of HepG2 using 66473-1-Ig

    WB analysis of HepG2 using 66473-1-Ig

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

  • WB analysis of HepG2 using 66473-1-Ig

    WB analysis of HepG2 using 66473-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 66473-1-Ig

    WB analysis of PC-3 using 66473-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66473-1-Ig

    IHC staining of human prostate cancer using 66473-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSAP antibody included
  • IHC staining of human prostate cancer using 66473-1-Ig

    IHC staining of human prostate cancer using 66473-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSAP antibody included
  • IF Staining of human prostate cancer using 66473-1-Ig

    IF Staining of human prostate cancer using 66473-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate cancer using 66473-1-Ig

    IF Staining of human prostate cancer using 66473-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman milk tissue, human milk, HepG2 cells, PC-3 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman prostate cancer tissue
Planning an IHC experiment? We recommend our IHCeasy PSAP Ready-To-Use IHC Kit. PSAP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66473-1-Ig targets PSAP in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSAP fusion protein Ag1194 相同性解析による交差性が予測される生物種
Full Name prosaposin
Calculated molecular weight 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC001503
Gene symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_2881839
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 66473-1-IgDownload protocol
IHC protocol for PSAP antibody 66473-1-IgDownload protocol
IF protocol for PSAP antibody 66473-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols