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Validation Data Gallery

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  • Western Blot (WB) analysis of human milk using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of human milk using 66473-1-Ig

    human milk were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HeLa using 66473-1-Ig

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HepG2 using 66473-1-Ig

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

  • Western Blot (WB) analysis of PC-3 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of PC-3 using 66473-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Monoclonal antibody (66473-1-Ig)

    WB analysis of HepG2 using 66473-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IHC staining of human prostate cancer using 66473-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IHC staining of human prostate cancer using 66473-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of human prostate cancer using 66473-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of human prostate cancer using 66473-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSAP Monoclonal antibody (66473-1-Ig)

    IF Staining of HeLa using 66473-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

PSAP Monoclonal antibody

PSAP Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66473-1-Ig
Clone No.2F11B2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CSAct, Component C, Co beta glucosidase, Cerebroside sulfate activator, A1 activator

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman milk tissue, human milk, HepG2 cells, PC-3 cells, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66473-1-Ig targets PSAP in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1194

Product name: Recombinant human PSAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC001503

Sequence: MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEF

相同性解析による交差性が予測される生物種
Full Name prosaposin
Calculated molecular weight 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC001503
Gene Symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_2881839
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP07602
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 66473-1-IgDownload protocol
IHC protocol for PSAP antibody 66473-1-IgDownload protocol
IF protocol for PSAP antibody 66473-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols