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  • Featured Product
  • KD/KO Validated

PCCB Monoclonal antibody

PCCB Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

pig, rat, mouse, human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A9A5

Cat no : 66501-1-Ig

Synonyms

PCCase subunit beta, PCCB

Validation Data Gallery

Applications:
  • WB analysis using 66501-1-Ig

    WB analysis using 66501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66501-1-Ig

    WB analysis using 66501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66501-1-Ig

    WB analysis of HepG2 using 66501-1-Ig

    WB result of PCCB antibody (66501-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCCB transfected HepG2 cells.

  • IHC staining of human heart using 66501-1-Ig

    IHC staining of human heart using 66501-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 10x lens).

  • IHC staining of human heart using 66501-1-Ig

    IHC staining of human heart using 66501-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 40x lens).

  • IF Staining of human kidney using 66501-1-Ig

    IF Staining of human kidney using 66501-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66501-1-Ig (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, human heart tissue, HepG2 cells, HSC-T6 cells, pig heart tissue, rat heart tissue, mouse liver tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66501-1-Ig targets PCCB in WB, IF, IHC, ELISA applications and shows reactivity with pig, rat, mouse, human samples.

Tested Reactivity pig, rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCCB fusion protein Ag1608 相同性解析による交差性が予測される生物種
Full Name propionyl Coenzyme A carboxylase, beta polypeptide
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC013768
Gene symbol PCCB
Gene ID (NCBI) 5096
RRIDAB_2881865
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCCB antibody 66501-1-IgDownload protocol
IHC protocol for PCCB antibody 66501-1-IgDownload protocol
IF protocol for PCCB antibody 66501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols