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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis using 66501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis using 66501-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PCCB Monoclonal antibody (66501-1-Ig)

    WB analysis of HepG2 using 66501-1-Ig

    WB result of PCCB antibody (66501-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCCB transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human heart tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IHC staining of human heart using 66501-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IHC staining of human heart using 66501-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IF Staining of mouse kidney using 66501-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PCCB antibody (66501-1-Ig, Clone: 1A9A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using PCCB Monoclonal antibody (66501-1-Ig)

    IF Staining of human kidney using 66501-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66501-1-Ig (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Affinity and Kinetic Characterization of 66501-1-Ig

    Affinity and Kinetic Characterization of 66501-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 66501-1-Ig against Human PCCB was performed. The affinity constant is 2.99 nM.

  • Featured Product
  • KD/KO Validated

PCCB Monoclonal antibody

PCCB Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66501-1-Ig
Clone No.1A9A5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

1A9A5, EC:6.4.1.3, PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, Propionyl-CoA carboxylase beta chain, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, human heart tissue, HepG2 cells, HSC-T6 cells, pig heart tissue, rat heart tissue, mouse liver tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue, human kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66501-1-Ig targets PCCB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag1608

Product name: Recombinant human PCCB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 240-539 aa of BC013768

Sequence: TQEELGGAKTHTTMSGVAHRAFENDVDALCNLRDFFNYLPLSSQDPAPVRECHDPSDRLVPELDTIVPLESTKAYNMVDIIHSVVDEREFFEIMPNYAKNIIVGFARMNGRTVGIVGNQPKVASGCLDINSSVKGARFVRFCDAFNIPLITFVDVPGFLPGTAQEYGGIIRHGAKLLYAFAEATVPKVTVITRKAYGGAYDVMSSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFANPFPAAVRGFVDDIIQPSSTRARICCDLDVLASKKVQRPWRKHANIPL

相同性解析による交差性が予測される生物種
Full Name propionyl Coenzyme A carboxylase, beta polypeptide
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC013768
Gene Symbol PCCB
Gene ID (NCBI) 5096
RRIDAB_2881865
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP05166
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PCCB antibody 66501-1-IgDownload protocol
IHC protocol for PCCB antibody 66501-1-IgDownload protocol
IF protocol for PCCB antibody 66501-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols