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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

P2RX5 Monoclonal antibody

P2RX5 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2A2A6

Cat no : 66430-1-Ig

Synonyms

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor

Validation Data Gallery

Applications:
  • WB analysis using 66430-1-Ig

    WB analysis using 66430-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66430-1-Ig (P2RX5 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig brain using 66430-1-Ig

    WB analysis of pig brain using 66430-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66430-1-Ig (P2RX5 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brown adipose using 66430-1-Ig

    IF Staining of mouse brown adipose using 66430-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using P2RX5 antibody (66430-1-Ig, Clone: 2A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of Jurkat using 66430-1-Ig

    FC experiment of Jurkat using 66430-1-Ig

    1X10^6 Jurkat cells were stained with 0.20 ug Anti-Human P2RX5 (66430-1-Ig, Clone:2A2A6) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inHEK-293 cells, pig brain tissue, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue
Positive IF detected inmouse brown adipose tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66430-1-Ig targets P2RX5 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P2RX5 fusion protein Ag5297 相同性解析による交差性が予測される生物種
Full Name purinergic receptor P2X, ligand-gated ion channel, 5
Calculated molecular weight 422 aa, 47 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC039015
Gene symbol P2RX5
Gene ID (NCBI) 5026
RRIDAB_2881801
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for P2RX5 antibody 66430-1-IgDownload protocol
IF protocol for P2RX5 antibody 66430-1-IgDownload protocol
FC protocol for P2RX5 antibody 66430-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols