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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ORM1/2 Monoclonal antibody

ORM1/2 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5F2H7

Cat no : CL488-66097

Synonyms

AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-66097

    IF Staining of HepG2 using CL488-66097

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66097 (ORM1/2 antibody) at dilution of 1:50.

  • IF Staining of mouse liver using CL488-66097

    IF Staining of mouse liver using CL488-66097

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 ORM1/2 antibody (CL488-66097, Clone: 5F2H7 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66097

    FC experiment of HepG2 using CL488-66097

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ORM1/2 (CL488-66097, Clone:5F2H7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells, mouse liver tissue
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66097-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66097 targets ORM1/2 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ORM1/2 fusion protein Ag19248 相同性解析による交差性が予測される生物種
Full Name orosomucoid 1
Calculated molecular weight 201 aa, 24 kDa
Observed molecular weight 40-47 kDa
GenBank accession numberBC026238
Gene symbol ORM1
Gene ID (NCBI) 5004
RRIDAB_2883258
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ORM1/2 antibody CL488-66097Download protocol
FC protocol for CL Plus 488 ORM1/2 antibody CL488-66097Download protocol
Standard Protocols
Click here to view our Standard Protocols