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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Nurr1/NR4A2 Monoclonal antibody

Nurr1/NR4A2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H5B4

Cat no : CL488-66878

Synonyms

HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-66878

    FC experiment of HeLa using CL488-66878

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Nurr1/NR4A2 (CL488-66878, Clone:1H5B4) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NR4A2 Ready-To-Use IHC Kit. NR4A2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66878-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66878 targets Nurr1/NR4A2 in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nurr1/NR4A2 fusion protein Ag1418 相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 4, group A, member 2
Calculated molecular weight 66 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC009288
Gene symbol NR4A2
Gene ID (NCBI) 4929
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Nurr1/NR4A2 antibody CL488-66878Download protocol
Standard Protocols
Click here to view our Standard Protocols