CoraLite® Plus 488-conjugated NIT2 Monoclonal antibody
NIT2 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, pig
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E5B1
Cat no : CL488-68169
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | U-251 cells |
For other applications, we recommend the unconjugated version of this antibody, 68169-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-68169 targets NIT2 in IF applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NIT2 fusion protein Ag9889 相同性解析による交差性が予測される生物種 |
| Full Name | nitrilase family, member 2 |
| Calculated molecular weight | 276 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC020620 |
| Gene symbol | NIT2 |
| Gene ID (NCBI) | 56954 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NIT2 antibody CL488-68169 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
