Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, pig liver tissue, mouse liver tissue, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
68169-1-Ig targets NIT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9889 Product name: Recombinant human NIT2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-276 aa of BC020620 Sequence: MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIPGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEALAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMKKP 相同性解析による交差性が予測される生物種 |
| Full Name | nitrilase family, member 2 |
| Calculated molecular weight | 276 aa, 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC020620 |
| Gene Symbol | NIT2 |
| Gene ID (NCBI) | 56954 |
| RRID | AB_2923685 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NQR4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NIT2 belongs to a branch of the nitrilase superfamily of enzymes that cleave carbon-nitrogen bonds. NIT2 functions as an omega-amidase and catalyzes hydrolysis of alpha-ketoglutaramate, forming alpha-ketoglutarate and ammonia. This reaction is also functionally coupled with a subset of transaminases that reaminate the keto acid analogs of some essential amino acids, most particularly methionine and phenylalanine.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NIT2 antibody 68169-1-Ig | Download protocol |
| IHC protocol for NIT2 antibody 68169-1-Ig | Download protocol |
| WB protocol for NIT2 antibody 68169-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |