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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated NEU1 Monoclonal antibody

NEU1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F6D1

Cat no : CL488-67032

Synonyms

Acetylneuraminyl hydrolase, FLJ93471, G9 sialidase, Lysosomal sialidase, N acetyl alpha neuraminidase 1, NANH, NEU, NEU1, NEURAMINIDASE 1, SIAL1, Sialidase 1

Validation Data Gallery

Applications:
  • IF Staining of U-251 using CL488-67032

    IF Staining of U-251 using CL488-67032

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 NEU1 antibody (CL488-67032, Clone: 1F6D1 ) at dilution of 1:200.

Tested Applications

Positive IF detected inU-251 cells
For other applications, we recommend the unconjugated version of this antibody, 67032-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67032 targets NEU1 in IF applications and shows reactivity with Human, Rat, Pig samples.

Tested Reactivity Human, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEU1 fusion protein Ag27945 相同性解析による交差性が予測される生物種
Full Name sialidase 1 (lysosomal sialidase)
Calculated molecular weight 45 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC011900
Gene symbol NEU1
Gene ID (NCBI) 4758
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEU1 (lysosomal sialidase) encodes a kind of lysosomal enzyme that could cleave terminal silic acids from glycoproteins or glycolipids to regulate various biological processes by conformational change (PMID:28130415). In the lysosome, NEU1 is one member of a heterotrimeric complex, the others are beta-galactosidase and cathepsin A. The NEU1 is widely expressed in mammalian tissues and involved in lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells (PMID:19075514). NUE1 also expresses on the plasma membrane where it modulates several signalling molecules about inflamation, exocytosis, phagocytosis, cell adhesion and proliferation (PMID:21928149). Addition, it is reported that mutation in NEU1 of human could lead to sialidosis (PMID:14517945).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NEU1 antibody CL488-67032Download protocol
Standard Protocols
Click here to view our Standard Protocols