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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MYH1-motor domain Polyclonal antibody

MYH1-motor domain Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22282-1-AP

Synonyms

MYH1, MYH1-motor domain, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d

Validation Data Gallery

Applications:
  • WB analysis of human skeletal muscle using 22282-1-AP

    WB analysis of human skeletal muscle using 22282-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22282-1-AP (MYH1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22282-1-AP

    IHC staining of human skeletal muscle using 22282-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MYH1 antibody included
  • IHC staining of human skeletal muscle using 22282-1-AP

    IHC staining of human skeletal muscle using 22282-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22282-1-AP (MYH1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MYH1 antibody included
  • FC experiment of C2C12 using 22282-1-AP

    FC experiment of C2C12 using 22282-1-AP

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYH1-motor domain (22282-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

22282-1-AP targets MYH1-motor domain in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name myosin, heavy chain 1, skeletal muscle, adult
Calculated molecular weight 223 kDa
Observed molecular weight 223 kDa
GenBank accession numberNM_005963
Gene symbol MYH1
Gene ID (NCBI) 4619
RRIDAB_2879059
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

Protocols

Product Specific Protocols
WB protocol for MYH1-motor domain antibody 22282-1-APDownload protocol
IHC protocol for MYH1-motor domain antibody 22282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Moonhee (Verified Customer) (11-18-2021)

It works very well. it can be diluted more for IF.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: RCC cells