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Species Reactivity
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MEST Monoclonal antibody

MEST Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A4A1

Cat no : 60263-1-Ig

Synonyms

MEST, PEG1

Validation Data Gallery

Applications:
  • WB analysis of PC-3 using 60263-1-Ig

    WB analysis of PC-3 using 60263-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 60263-1-Ig

    IHC staining of human prostate cancer using 60263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens).

    Ready-to-use IHC kits available with MEST antibody included
  • IHC staining of human prostate cancer using 60263-1-Ig

    IHC staining of human prostate cancer using 60263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens).

    Ready-to-use IHC kits available with MEST antibody included
  • IF Staining of BxPC-3 using 60263-1-Ig

    IF Staining of BxPC-3 using 60263-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy MEST Ready-To-Use IHC Kit. MEST primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60263-1-Ig is MEST Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MEST fusion protein Ag11929 相同性解析による交差性が予測される生物種
Full Name mesoderm specific transcript homolog (mouse)
Calculated molecular weight 38 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC011908
Gene symbol MEST
Gene ID (NCBI) 4232
RRIDAB_2881384
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.

Protocols

Product Specific Protocols
WB protocol for MEST antibody 60263-1-IgDownload protocol
IHC protocol for MEST antibody 60263-1-IgDownload protocol
IF protocol for MEST antibody 60263-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols