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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using MEST Monoclonal antibody (60263-1-Ig)

    WB analysis of PC-3 using 60263-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60263-1-Ig (MEST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MEST Monoclonal antibody (60263-1-Ig)

    IHC staining of human prostate cancer using 60263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MEST Monoclonal antibody (60263-1-Ig)

    IHC staining of human prostate cancer using 60263-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60263-1-Ig (MEST Antibody) at dilution of 1:400 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using MEST Monoclonal antibody (60263-1-Ig)

    IF Staining of BxPC-3 using 60263-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed BxPC-3 cells using 60263-1-Ig(MEST antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

MEST Monoclonal antibody

MEST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60263-1-Ig
Clone No.1A4A1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1A4A1, EC:3.-.-.-, Mesoderm-specific transcript homolog protein, Paternally-expressed gene 1 protein, PEG1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60263-1-Ig targets MEST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11929

Product name: Recombinant human MEST protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-234 aa of BC011908

Sequence: MVRRDRLRRMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPILTRLMNFFVFSRGLTPVFGPYTRPSESELWD

相同性解析による交差性が予測される生物種
Full Name mesoderm specific transcript homolog (mouse)
Calculated molecular weight 38 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC011908
Gene Symbol MEST
Gene ID (NCBI) 4232
RRIDAB_2881384
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ5EB52
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEST(Mesoderm-specific transcript homolog protein) is also named as PEG1 and belongs to the AB hydrolase superfamily. The gene is a imprinting gene, which is associated with growth of mesodermal origin cells(PMID:20339302) and plays important roles in embryo development(PMID:21375506). It is also a novel regulator of Wnt/CTNNB signalling during adipogenic differentiation(PMID:21375506). It has 3 isoforms produced by alternative splicing and the full length protein has a glycosylation site.

Protocols

Product Specific Protocols
WB protocol for MEST antibody 60263-1-IgDownload protocol
IHC protocol for MEST antibody 60263-1-IgDownload protocol
IF protocol for MEST antibody 60263-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols