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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated LATS1 Polyclonal antibody

LATS1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17049

Synonyms

h warts, LATS1, WARTS, WARTS protein kinase, wts

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-17049

    IF Staining of HeLa using CL488-17049

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 LATS1 antibody (CL488-17049) at dilution of 1:200.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 17049-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-17049 targets LATS1 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LATS1 fusion protein Ag10709 相同性解析による交差性が予測される生物種
Full Name LATS, large tumor suppressor, homolog 1 (Drosophila)
Calculated molecular weight 15 kDa, 127 kDa
Observed molecular weight 140-150 kDa, 120 kDa
GenBank accession numberBC015665
Gene symbol LATS1
Gene ID (NCBI) 9113
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongs to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 LATS1 antibody CL488-17049Download protocol
Standard Protocols
Click here to view our Standard Protocols