human IgD Monoclonal antibody

human IgD Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D1B12

Cat no : 67538-1-Ig

Synonyms

FLJ00382, FLJ46727, IGHD



Tested Applications

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

67538-1-Ig targets human IgD in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen human IgD fusion protein Ag9614 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant delta
Calculated molecular weight 573 aa, 63 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC021276
Gene symbol IGHD
Gene ID (NCBI) 3495
RRIDAB_2882757
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .

Protocols

Product Specific Protocols
WB protocol for human IgD antibody 67538-1-IgDownload protocol
IHC protocol for human IgD antibody 67538-1-IgDownload protocol
IF protocol for human IgD antibody 67538-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Exp Mol Med

Delineating the dynamic evolution from preneoplasia to invasive lung adenocarcinoma by integrating single-cell RNA sequencing and spatial transcriptomics

Authors - Jianfei Zhu