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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using human IgD Monoclonal antibody (67538-1-Ig)

    WB analysis of human plasma using 67538-1-Ig (same clone as 67538-1-PBS)

    human plasma (0.1 ul) were subjected to SDS PAGE followed by western blot with 67538-1-Ig (human IgD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgD Monoclonal antibody (67538-1-Ig)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgD Monoclonal antibody (67538-1-Ig)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgD Monoclonal antibody (67538-1-Ig)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using human IgD Monoclonal antibody (67538-1-Ig)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using human IgD Monoclonal antibody (67538-1-Ig)

    IF Staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IGHD antibody (67538-1-Ig, Clone: 1D1B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

human IgD Monoclonal antibody, PBS Only

human IgD Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67538-1-PBS
Clone No.1D1B12

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

IGHD, IgD, 1D1B12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67538-1-PBS targets human IgD in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9614

Product name: Recombinant human IGHD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 202-548 aa of BC021276

Sequence: RRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYLAMTPLIPQSKDENSDDYTTFDDVGSLWT

相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant delta
Calculated molecular weight 573 aa, 63 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC021276
Gene Symbol IGHD
Gene ID (NCBI) 3495
RRIDAB_2882757
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .