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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using HRP-conjugated human IgD Monoclonal antibody (HRP-67538)

    WB analysis of human plasma using HRP-67538

    human plasma were subjected to SDS PAGE followed by western blot with HRP-67538 (human IgD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HRP-conjugated IgD Monoclonal antibody

human IgD Monoclonal Antibody for WB
Cat No. HRP-67538
Clone No.1D1B12

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB

Synonyms

FLJ00382, FLJ46727, IGHD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  HRP
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

HRP-67538 targets human IgD in WB applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9614

Product name: Recombinant human IGHD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 202-548 aa of BC021276

Sequence: RRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYLAMTPLIPQSKDENSDDYTTFDDVGSLWT

相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant delta
Calculated molecular weight 573 aa, 63 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC021276
Gene Symbol IGHD
Gene ID (NCBI) 3495
RRIDAB_2920512
Conjugate HRP
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .

Protocols

Product Specific Protocols
WB protocol for HRP human IgD antibody HRP-67538Download protocol
Standard Protocols
Click here to view our Standard Protocols