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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Fibronectin Monoclonal antibody

Fibronectin Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G10F9

Cat no : CL594-66042

Synonyms

CIG, Cold insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF

Validation Data Gallery

Applications:
  • IF Staining of human colon cancer using CL594-66042

    IF Staining of human colon cancer using CL594-66042

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Fibronectin antibody (CL594-66042, Clone: 1G10F9 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL594-66042

    IF Staining of human colon cancer using CL594-66042

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Fibronectin antibody (CL594-66042, Clone: 1G10F9 ) at dilution of 1:200.

  • IF Staining of NIH/3T3 using CL594-66042

    IF Staining of NIH/3T3 using CL594-66042

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CL594-66042 (Fibronectin antibody) at dilution of 1:100.

  • FC experiment of HEK-293 using CL594-66042

    FC experiment of HEK-293 using CL594-66042

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Fibronectin (CL594-66042, Clone:1G10F9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inhuman colon cancer tissue, NIH/3T3 cells
Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66042-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66042 is Fibronectin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Fibronectin fusion protein Ag8016 相同性解析による交差性が予測される生物種
Full Name fibronectin 1
Calculated molecular weight 2386 aa, 263 kDa
Observed molecular weight 263 kDa
GenBank accession numberBC005858
Gene symbol FN1
Gene ID (NCBI) 2335
RRIDAB_2883488
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibronectin 1 (FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma (plasma FN1) and other body fluids and an insoluble form in the extracellular matrix (cellular FN1). Plasma FN1 (dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis. (PMID: 7276612; 12244123; 12847088)

Protocols

Product Specific Protocols
IF protocol for CL594 Fibronectin antibody CL594-66042Download protocol
FC protocol for CL594 Fibronectin antibody CL594-66042Download protocol
Standard Protocols
Click here to view our Standard Protocols