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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ER Polyclonal antibody

ER Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21244

Synonyms

DKFZp686N23123, ER, ER alpha, Era, ESR, ESR1, ESRA, Estradiol receptor, Estrogen receptor, estrogen receptor 1, NR3A1

Validation Data Gallery

Applications:
  • FC experiment of MCF-7 using CL488-21244

    FC experiment of MCF-7 using CL488-21244

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ER (CL488-21244) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 21244-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-21244 targets ER in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ER fusion protein Ag15738 相同性解析による交差性が予測される生物種
Full Name estrogen receptor 1
Calculated molecular weight 595 aa, 66 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC128573
Gene symbol ESR1
Gene ID (NCBI) 2099
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ER antibody CL488-21244Download protocol
Standard Protocols
Click here to view our Standard Protocols