• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10944

Synonyms

Corticoliberin, Corticotropin releasing factor, CRF, CRH

Validation Data Gallery

Applications:
  • IF Staining of mouse brain using CL488-10944

    IF Staining of mouse brain using CL488-10944

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 CRH/CRF antibody (CL488-10944) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-10944

    IF Staining of mouse brain using CL488-10944

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 CRH/CRF antibody (CL488-10944) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 10944-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-10944 targets CRH/CRF in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRH/CRF fusion protein Ag1371 相同性解析による交差性が予測される生物種
Full Name CRH/CRF
Calculated molecular weight 21 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC011031
Gene symbol CRH
Gene ID (NCBI) 1392
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CRH/CRF antibody CL488-10944Download protocol
Standard Protocols
Click here to view our Standard Protocols