Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 19 publications below |
| IHC | See 14 publications below |
| IF | See 21 publications below |
Product Information
10944-1-AP targets CRH/CRF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish, brandt’s voles, microtus ochrogaster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1371 Product name: Recombinant human CRH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-196 aa of BC011031 Sequence: MRLPLLVSAGVLLVALLPCPPCRALLSRGPVPGARQAPQHPQPLDFFQPPPQSEQPQQPQARPVLLRMGEEYFLRLGNLNKSPAAPLSPASSLLAGGSGSRPSPEQATANFFRVLLQQLLLPRRSLDSPAALAERGARNALGGHQEAPERERRSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEIIGK 相同性解析による交差性が予測される生物種 |
| Full Name | CRH/CRF |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 35-38 kDa |
| GenBank accession number | BC011031 |
| Gene Symbol | CRH |
| Gene ID (NCBI) | 1392 |
| RRID | AB_2084279 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06850 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.
Publications
| Species | Application | Title |
|---|---|---|
Cell Res 40 Hz light flickering alleviates chronic pain via adenosine signaling in the retina-amygdala pathway. | ||
Cell Stem Cell Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells. | ||
Nat Commun Paraventricular hypothalamic RUVBL2 neurons suppress appetite by enhancing excitatory synaptic transmission in distinct neurocircuits | ||
J Adv Res Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system. | ||
Neuron Corticotropin-releasing hormone signaling from piriform cortex to amygdala mediates social homophily in opioid addiction | ||
J Exp Med Central neurons encode interleukin-1β signals and mediate stress-induced inflammation. |