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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CNPY2, MSAP Polyclonal antibody

CNPY2, MSAP Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14635

Synonyms

canopy 2 homolog (zebrafish), CNPY2, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9

Validation Data Gallery

Applications:
  • FC experiment of HepG2 using CL488-14635

    FC experiment of HepG2 using CL488-14635

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CNPY2, MSAP (CL488-14635) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 14635-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-14635 targets CNPY2, MSAP in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNPY2, MSAP fusion protein Ag6214 相同性解析による交差性が予測される生物種
Full Name canopy 2 homolog (zebrafish)
Calculated molecular weight 21 kDa
Observed molecular weight 17-21 kDa
GenBank accession numberBC065015
Gene symbol CNPY2
Gene ID (NCBI) 10330
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CNPY2, MSAP antibody CL488-14635Download protocol
Standard Protocols
Click here to view our Standard Protocols