Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of mouse pancreas using 14635-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 14635-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14635-1-AP
WB result of CNPY2, MSAP antibody (14635-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPY2, MSAP transfected HepG2 cells.
WB analysis of human liver using 14635-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 14635-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 14635-1-AP
IP result of anti-CNPY2, MSAP (IP:14635-1-AP, 3ug; Detection:14635-1-AP 1:500) with HepG2 cells lysate 600ug.
IHC staining of human liver using 14635-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IHC staining of human liver using 14635-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14635-1-AP (CNPY2, MSAP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IHC staining of human pancreas cancer using 14635-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14635-1-AP (CNPY2,MSAP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14635-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14635-1-AP (CNPY2,MSAP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14635-1-AP
Immunofluorescent analysis of HepG2 cells using 14635-1-AP (CNPY2,MSAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 14635-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CNPY2, MSAP antibody (14635-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
FC experiment of HepG2 using 14635-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CNPY2, MSAP (14635-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

CNPY2/MSAP Polyclonal antibody

CNPY2/MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14635-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

CNPY2, CNPY2, MSAP, CNPY2,MSAP, canopy 2 homolog (zebrafish), HP10390

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, mouse pancreas tissue, human liver tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 15 publications below
IHC	See 5 publications below
IF	See 4 publications below

Product Information

14635-1-AP targets CNPY2/MSAP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6214 Product name: Recombinant human CNPY2,MSAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-182 aa of BC065015 Sequence: MKGWGWLALLLGALLGTAWARRSQDLHCGACRALVDELEWEIAQVDPKKTIQMGSFRINPDGSQSVVEVPYARSEAHLTELLEEICDRMKEYGEQIDPSTHRKNYVRVVGRNGESSELDLQGIRIDSDISGTLKFACESIVEEYEDELIEFFSREADNVKDKLCSKRTDLCDHALHISHDEL 相同性解析による交差性が予測される生物種
Full Name	canopy 2 homolog (zebrafish)
Calculated molecular weight	21 kDa
Observed molecular weight	17-21 kDa
GenBank accession number	BC065015
Gene Symbol	CNPY2
Gene ID (NCBI)	10330
RRID	AB_2082621
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y2B0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2.

Protocols

Product Specific Protocols
IF protocol for CNPY2/MSAP antibody 14635-1-AP	Download protocol
IHC protocol for CNPY2/MSAP antibody 14635-1-AP	Download protocol
IP protocol for CNPY2/MSAP antibody 14635-1-AP	Download protocol
WB protocol for CNPY2/MSAP antibody 14635-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB	Nat Struct Mol Biol CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response. Authors - Feng Hong KO Validated View Article
mouse	IHC	Cancers (Basel) Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis. Authors - Anna Kakehashi View Article
human	WB,IF	Apoptosis Angiotensin-(1-9) prevents angiotensin II-induced endothelial apoptosis through CNPY2/PERK pathway Authors - Chun-Ling Guo KD Validated View Article
rat	WB	J Cell Mol Med PMID: 31625681 Authors - Lina Zhao View Article
mouse	WB	J Biol Chem Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap). Authors - Do Hai Thi HT KD Validated View Article
rat	WB	J Biol Chem Reciprocal regulation of Very low-density lipoprotein receptors (VLDLRs) in neurons by Brain-derived neurotrophic factor (BDNF) and Reelin: Involvement of the E3 ligase Mylip/Idol. Authors - Do Hai Thi HT View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse pancreas using 14635-1-AP

WB analysis using 14635-1-AP

WB analysis of HepG2 using 14635-1-AP

WB analysis of human liver using 14635-1-AP

WB analysis of mouse pancreas using 14635-1-AP

IP experiment of HepG2 using 14635-1-AP

IHC staining of human liver using 14635-1-AP

IHC staining of human liver using 14635-1-AP

IHC staining of human pancreas cancer using 14635-1-AP

IHC staining of human pancreas cancer using 14635-1-AP

IF Staining of HepG2 using 14635-1-AP

IF Staining of HepG2 using 14635-1-AP

FC experiment of HepG2 using 14635-1-AP

CNPY2/MSAP Polyclonal antibody

CNPY2/MSAP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CNPY2 is a key initiator of the PERK-CHOP pathway of the unfolded protein response.

Canopy Homolog 2 as a Novel Molecular Target in Hepatocarcinogenesis.

Angiotensin-(1-9) prevents angiotensin II-induced endothelial apoptosis through CNPY2/PERK pathway

PMID: 31625681

Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap).

Reciprocal regulation of Very low-density lipoprotein receptors (VLDLRs) in neurons by Brain-derived neurotrophic factor (BDNF) and Reelin: Involvement of the E3 ligase Mylip/Idol.