CoraLite® Plus 488-conjugated AIFM2/ FSP1 Monoclonal antibody
AIFM2/ FSP1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A2B2
Cat no : CL488-68049
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive FC detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68049-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-68049 targets AIFM2/ FSP1 in FC (Intra) applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | AIFM2/ FSP1 fusion protein Ag30516 相同性解析による交差性が予測される生物種 |
| Full Name | apoptosis-inducing factor, mitochondrion-associated, 2 |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 41 kDa |
| GenBank accession number | BC023601 |
| Gene symbol | AIFM2 |
| Gene ID (NCBI) | 84883 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 AIFM2/ FSP1 antibody CL488-68049 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
