1X10^6 CHO TIM3 stable expressing cells (red) and CHO parental cells were surface stained with 0.5 µg CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt). Cells were not fixed.
1X10^6 CHO TIM3 stable expressing cells (red) were surface stained with 0.5 µg CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt). Cells were not fixed and pre-gated on co-expressed, GFP positive cells.
Live HeLa cells transfected with human TIM-3 were immunostained with CoraLite® Plus 555 conjugated-TIM3 VHH (CL555-tmt,1:1000, yellow). After formaldehyde fixation, triton permeabilization and blocking with BSA, intracellular pool of TIM-3 was immunostained with CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt, 1:1000, magenta). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed.
Live HeLa cells transfected with human TIM-3 were immunostained with CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt, 1:1000, magenta). Cells were fixed and nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with 20x objective and post-processed.
Note: Immunostaining with CL647-tmt can also be performed after formaldehyde fixation of the cells.
Live HeLa cells transfected with human TIM-3 were probed with cobolimab biosimilar (anti-TIM3, huIgG4). After formaldehyde fixation, the cells were immunostained with CoraLite® Plus 647 conjugated-TIM3 VHH (CL647-tmt, 1:1000, magenta) and with anti-human IgG Nano-Secondary (shuGCL488-2, green). Cell nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with 20x objective and post-processed.
