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Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of Daudi using 60355-1-Ig (same clone as 60355-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig thymus tissue using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of pig thymus using 60355-1-Ig (same clone as 60355-1-PBS)

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ROS1728 cells using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of ROS1728 using 60355-1-Ig (same clone as 60355-1-PBS)

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of human heart using 60355-1-Ig (same clone as 60355-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of RAW 264.7 using 60355-1-Ig (same clone as 60355-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of rat heart using 60355-1-Ig (same clone as 60355-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using TIM3 Monoclonal antibody (60355-1-Ig)

    WB analysis of PC-3 using 60355-1-Ig (same clone as 60355-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60355-1-Ig (TIM3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60355-1-PBS in a different storage buffer formulation.

TIM3 Monoclonal antibody, PBS Only

TIM3 Monoclonal Antibody for WB, ELISA
Cat No. 60355-1-PBS
Clone No.4C4G3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

4C4G3, CD366, HAVCR2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60355-1-PBS targets TIM3 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16901

Product name: Recombinant human HAVCR2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-142 aa of BC020843

Sequence: MFSHLPFDCVLLLLLLLLTRSSEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPGEWTFACHLYE

相同性解析による交差性が予測される生物種
Full Name hepatitis A virus cellular receptor 2
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight 33 kDa, 50-70 kDa
GenBank accession numberBC020843
Gene Symbol TIM3
Gene ID (NCBI) 84868
RRIDAB_2881464
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8TDQ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).