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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human GDNF protein

Activity

<10 ng/mL

Specific Activity

minimally 1.00 x 106 IU/mg

Species Reactivity

human

Purity

>95%

Publications(1)

Cat no : HZ-1311

Synonyms

ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF

Validation Data Gallery

  • Purity of recombinant human GDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human GDNF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • iPSC-derived Human Midbrain Organoids Cultured using Recombinant Human Noggin, Sonic Hedgehog (SHH), FGF-8B, GDNF and BDNF). iPSCs differentiated to Midbrain organoids using HumanKine® recombinant human Noggin (HZ-1118), HumanKine® recombinant human SHH (HZ-1306), HumanKine® recombinant human FGF-8b (HZ-1103), HumanKine® recombinant human GDNF ( HZ-1311), HumanKine® recombinant human BDNF ( HZ-1335) were fixed and permeabilized for detection of endogenous SOX2 and Nestin (a) using SOX2 Monoclonal antibody (Product # 66411-1-Ig, 1:400 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)), and Nestin Polyclonal antibody (Product # 19483-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) and (b) detection of endogenous MASH1 and MAP2 ASCL1 (MASH1) Polyclonal antibody (Product # 31206-1-AP, 1:200 dilution,  labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)) and MAP2 Monoclonal antibody (Product # 67015-1-Ig, 1:2000 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)). (c and d) and detection of endogenous TH and DAT using TH Polyclonal antibody (Product #25859-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) ) and Dopamine Transporter/DAT Polyclonal antibody (Product # 22524-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)).

    iPSC-derived Human Midbrain Organoids Cultured using Recombinant Human Noggin, Sonic Hedgehog (SHH), FGF-8B, GDNF and BDNF). iPSCs differentiated to Midbrain organoids using HumanKine® recombinant human Noggin (HZ-1118), HumanKine® recombinant human SHH (HZ-1306), HumanKine® recombinant human FGF-8b (HZ-1103), HumanKine® recombinant human GDNF ( HZ-1311), HumanKine® recombinant human BDNF ( HZ-1335) were fixed and permeabilized for detection of endogenous SOX2 and Nestin (a) using SOX2 Monoclonal antibody (Product # 66411-1-Ig, 1:400 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)), and Nestin Polyclonal antibody (Product # 19483-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) and (b) detection of endogenous MASH1 and MAP2 ASCL1 (MASH1) Polyclonal antibody (Product # 31206-1-AP, 1:200 dilution,  labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)) and MAP2 Monoclonal antibody (Product # 67015-1-Ig, 1:2000 dilution, labelled with Multi-rAb™ CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004)). (c and d) and detection of endogenous TH and DAT using TH Polyclonal antibody (Product #25859-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002) ) and Dopamine Transporter/DAT Polyclonal antibody (Product # 22524-1-AP, 1:200 dilution, labelled with Multi-rAb® CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)).

  • GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

    GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

  • Proteintech GDNF (HZ-1311) demonstrates a greater fold induction of proliferation compared to a leading competitor at an equivalent EC50. GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

    Proteintech GDNF (HZ-1311) demonstrates a greater fold induction of proliferation compared to a leading competitor at an equivalent EC50. GDNF stimulates dose-dependent proliferation of the SH-SY5Y Neuroblastoma cell line. SH-SY5Y cells were treated with increasing concentrations of recombinant GDNF for 72 hours in the presence of 100 ng/mL GFR alpha-1. Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is < 10 ng/mL.

  • Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta Johannes Kepler Universitat)

    Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta Johannes Kepler Universitat)

Technical Specifications

GeneID 2668
Species Human
Expression HEK293
Activity <10 ng/mL
Specific Activityminimally 1.00 x 106 IU/mg
Purity >95%
Endotoxin<1 EU/g
Accession NumberP39905
Molecular Mass 22 to 25 kDa reduced, 30 to 38 kDa non-reduced, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

HumanKine® Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a potent neurotrophic factor that promotes the survival, maintenance, and differentiation of neurons, particularly dopaminergic and motor neurons in the central and peripheral nervous systems. GDNF is widely used as a cell culture supplement for neural differentiation, stem cell research, and brain organoid development. Produced in a HEK293 human expression system, HumanKine® GDNF closely mimics native human protein, delivering enhanced bioactivity and stability. GDNF signals through the GFRα1/RET receptor complex, supporting neuronal survival, neurite outgrowth, and synaptic function. GDNF plays a key role in neuroprotection and tissue regeneration and has been extensively studied in neurodegenerative diseases such as Parkinson’s disease and amyotrophic lateral sclerosis (ALS).

Synonyms

ATF, ATF1, ATF2, GDNF, HFB1 GDNF, hGDNF

Publications

SpeciesTitle

Reprod Biol Endocrinol

Expression of transcriptional factor EB (TFEB) in differentiating spermatogonia potentially promotes cell migration in mouse seminiferous epithelium.

Authors - Yue Liu
View Article
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