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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using p300 Recombinant antibody (83078-5-RR)

    IP experiment of HeLa using 83078-5-RR

    IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using p300 Recombinant antibody (83078-5-RR)

    IF Staining of A431 using 83078-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using p300 Recombinant antibody (83078-5-RR)

    IF Staining of HepG2 using 83078-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of THP-1 cells using p300 Recombinant monoclonal antibody (83078-5-RR)

    FC experiment of THP-1 using 83078-5-RR

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • ChIP experiment of K-562 cells using p300 Recombinant monoclonal antibody (83078-5-RR)

    ChIP experiment of K-562 using 83078-5-RR

    Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of p300 (83078-5-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

p300 Recombinant monoclonal antibody

p300 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR
Cat No. 83078-5-RR
Clone No.230369D12
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR

Synonyms

EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHeLa cells
Positive IF/ICC detected inA431 cells, HepG2 cells
Positive FC (Intra) detected inTHP-1 cells
Positive ChIP-qPCR detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

83078-5-RR targets p300 in WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name E1A binding protein p300
Calculated molecular weight 264 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_001429
Gene Symbol EP300
Gene ID (NCBI) 2033
RRIDAB_3670795
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ09472
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

Protocols

Product Specific Protocols
IF protocol for p300 antibody 83078-5-RRDownload protocol
IP protocol for p300 antibody 83078-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

GCLC desuccinylation regulated by oxidative stress protects human cancer cells from ferroptosis

Authors - Zixiang Chen
View Article
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