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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using p300 Recombinant antibody (83078-5-RR)

    IP experiment of HeLa using 83078-5-RR (same clone as 83078-5-PBS)

    IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using p300 Recombinant antibody (83078-5-RR)

    IF Staining of A431 using 83078-5-RR (same clone as 83078-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using p300 Recombinant antibody (83078-5-RR)

    IF Staining of HepG2 using 83078-5-RR (same clone as 83078-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using p300 Recombinant antibody (83078-5-RR)

    FC experiment of THP-1 using 83078-5-RR (same clone as 83078-5-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

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p300 Recombinant antibody, PBS Only

p300 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 83078-5-PBS
Clone No.230369D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

EP300, Histone butyryltransferase p300, EC:2.3.1.48, EC:2.3.1.-, E1A-associated protein p300

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83078-5-PBS targets p300 in IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name E1A binding protein p300
Calculated molecular weight 264 kDa
GenBank accession numberNM_001429
Gene Symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09472
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.