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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using p300 Recombinant antibody (83078-5-RR)

    IP experiment of HeLa using 83078-5-RR (same clone as 83078-5-PBS)

    IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using p300 Recombinant antibody (83078-5-RR)

    IF Staining of A431 using 83078-5-RR (same clone as 83078-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using p300 Recombinant antibody (83078-5-RR)

    IF Staining of HepG2 using 83078-5-RR (same clone as 83078-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of THP-1 cells using p300 Recombinant antibody (83078-5-RR)

    FC experiment of THP-1 using 83078-5-RR (same clone as 83078-5-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83078-5-PBS in a different storage buffer formulation.

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p300 Recombinant antibody, PBS Only

p300 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 83078-5-PBS
Clone No.230369D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83078-5-PBS targets p300 in IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name E1A binding protein p300
Calculated molecular weight 264 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_001429
Gene Symbol EP300
Gene ID (NCBI) 2033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09472
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).