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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using mCherry Polyclonal antibody (26765-1-AP)

    WB analysis using 26765-1-AP (same clone as 26765-1-PBS)

    mCherry tagged protein was subjected to SDS PAGE followed by western blot with 26765-1-AP (mCherry antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using mCherry Polyclonal antibody (26765-1-AP)

    WB analysis of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

    WB result of mCherry antibody (26765-1-AP; 1:2000; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using mCherry Polyclonal antibody (26765-1-AP)

    WB analysis of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

    WB result of mCherry antibody (26765-1-AP; 1:300; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293T cells using mCherry Polyclonal antibody (26765-1-AP)

    IP experiment of Transfected HEK-293T using 26765-1-AP (same clone as 26765-1-PBS)

    IP result of anti-mCherry (IP:26765-1-AP, 4ug; Detection:26765-1-AP 1:2000) with Transfected HEK-293T cells lysate 1280 ug. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using mCherry Polyclonal antibody (26765-1-AP)

    IF Staining of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (26765-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

mCherry Polyclonal antibody, PBS Only

mCherry Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 26765-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Red fluorescence protein, red fluorescent protein, RFP, RFP-tag

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26765-1-PBS targets mCherry in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25320

Product name: Recombinant mCherry protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-235 aa of

Sequence: VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK

相同性解析による交差性が予測される生物種
Full Name mCherry
Calculated molecular weight 27 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2876881
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.

The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm).

When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody raised against mCherry. It can be used to detect mCherry, dsRed, tdTomato, and mScarlet.