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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated hIST1 Monoclonal antibody (CL594-66989)

    IF Staining of HeLa using CL594-66989

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 hIST1 antibody (CL594-66989, Clone: 1E12A7 ) at dilution of 1:200.

CoraLite®594-conjugated hIST1 Monoclonal antibody

hIST1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66989
Clone No.1E12A7

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

hIST1, IST1, IST1 homolog, KIAA0174, OLC1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66989 targets hIST1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28364

Product name: Recombinant human hIST1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-360 aa of BC000430

Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESMTLMLIRISLLHRLLVLDPSQKPLQSFLPDLQITMTTLSYQSCHLCQTHYQLHLLVPAPQHLKTLTLMIFPGGLKS

相同性解析による交差性が予測される生物種
Full Name KIAA0174
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC000430
Gene Symbol hIST1
Gene ID (NCBI) 9798
RRIDAB_2920068
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP53990
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member.

Protocols

Product Specific Protocols
IF protocol for CL594 hIST1 antibody CL594-66989Download protocol
Standard Protocols
Click here to view our Standard Protocols