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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using hCG Alpha Recombinant monoclonal antibody (85800-9-RR)

    WB analysis using 85800-9-RR (same clone as 85800-9-PBS)

    Hydroxyurea treated JAR cells were subjected to SDS PAGE followed by western blot with 85800-9-RR (CGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85800-9-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Alpha Recombinant monoclonal antibody (85800-9-RR)

    IHC staining of human placenta using 85800-9-RR (same clone as 85800-9-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-9-RR (CGA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85800-9-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Alpha Recombinant monoclonal antibody (85800-9-RR)

    IHC staining of human placenta using 85800-9-RR (same clone as 85800-9-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-9-RR (CGA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85800-9-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of JAR cells using hCG Alpha Recombinant monoclonal antibody (85800-9-RR)

    IF Staining of JAR using 85800-9-RR (same clone as 85800-9-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Hydroxyurea treated JAR cells using hCG Alpha antibody (85800-9-RR, Clone: 250136C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85800-9-PBS in a different storage buffer formulation.

hCG Alpha Recombinant monoclonal antibody, PBS Only

hCG Alpha Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85800-9-PBS
Clone No.250136C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CGA, HCG, Anterior pituitary glycoprotein hormones common subunit alpha, CG ALPHA, CG-alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85800-9-PBS targets hCG Alpha in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3377

Product name: Recombinant Human hCG Alpha protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 25-116 aa of

Sequence: APDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

相同性解析による交差性が予測される生物種
Full Name hCG Alpha
Calculated molecular weight18 kDa
Observed molecular weight20 kDa
Gene Symbol
Gene ID (NCBI) 1082
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP0DN86(CGB)+P01215(CGA)
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone consisting of two non-covalently linked subunits: a common α-subunit (hCGα) and a hormone-specific β-subunit (hCGβ). The α-subunit, also designated CGA (chorionic gonadotropin alpha chain), is a 92-amino-acid glycopeptide of ~14-22 kDa that is identical to the α-subunits of the pituitary glycoprotein hormones luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). Because the α-subunit is shared among these hormones, its biological specificity is conferred by the unique β-subunit with which it associates.

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