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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using hCG Alpha Recombinant antibody (85800-9-RR)

    WB analysis using 85800-9-RR

    Hydroxyurea treated JAR cells were subjected to SDS PAGE followed by western blot with 85800-9-RR (CGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Alpha Recombinant antibody (85800-9-RR)

    IHC staining of human placenta using 85800-9-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-9-RR (CGA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using hCG Alpha Recombinant antibody (85800-9-RR)

    IHC staining of human placenta using 85800-9-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-9-RR (CGA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of JAR cells using hCG Alpha Recombinant monoclonal antibody (85800-9-RR)

    IF Staining of JAR using 85800-9-RR

    Immunofluorescent analysis of (4% PFA) fixed Hydroxyurea treated JAR cells using hCG Alpha antibody (85800-9-RR, Clone: 250136C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

hCG Alpha Recombinant monoclonal antibody

hCG Alpha Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85800-9-RR
Clone No.250136C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CGA, HCG, Anterior pituitary glycoprotein hormones common subunit alpha, CG ALPHA, CG-alpha

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHydroxyurea treated JAR cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHydroxyurea treated JAR cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85800-9-RR targets hCG Alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3377

Product name: Recombinant Human hCG Alpha protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 25-116 aa of

Sequence: APDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

相同性解析による交差性が予測される生物種
Full Name hCG Alpha
Calculated molecular weight18 kDa
Observed molecular weight20 kDa
Gene Symbol
Gene ID (NCBI) 1082
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP0DN86(CGB)+P01215(CGA)
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone consisting of two non-covalently linked subunits: a common α-subunit (hCGα) and a hormone-specific β-subunit (hCGβ). The α-subunit, also designated CGA (chorionic gonadotropin alpha chain), is a 92-amino-acid glycopeptide of ~14-22 kDa that is identical to the α-subunits of the pituitary glycoprotein hormones luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). Because the α-subunit is shared among these hormones, its biological specificity is conferred by the unique β-subunit with which it associates.

Protocols

Product Specific Protocols
IF protocol for hCG Alpha antibody 85800-9-RRDownload protocol
IHC protocol for hCG Alpha antibody 85800-9-RRDownload protocol
WB protocol for hCG Alpha antibody 85800-9-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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