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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cyclin B1 Monoclonal antibody (67686-1-Ig)

    WB analysis using 67686-1-Ig (same clone as 67686-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67686-1-Ig (cyclin B1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    WB analysis of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

    WB result of cyclin B1 antibody (67686-1-Ig; 1:3200; incubated at room temperature for 1.5 hours) with sh-Control and sh-cyclin B1 transfected HeLa cells. This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IHC staining of human tonsillitis using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IHC staining of human tonsillitis using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skin tissue using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IHC staining of rat skin using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67686-1-Ig (cyclin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IF Staining of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IF Staining of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:650 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    IF Staining of HT-29 using 67686-1-Ig (same clone as 67686-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using cyclin B1 antibody (67686-1-Ig, Clone: 1F3G11 ) at dilution of 1:650 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using cyclin B1 Monoclonal antibody (67686-1-Ig)

    FC experiment of HeLa using 67686-1-Ig (same clone as 67686-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug cyclin B1 Monoclonal antibody (67686-1-Ig, Clone:1F3G11)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67686-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

cyclin B1 Monoclonal antibody, PBS Only

cyclin B1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67686-1-PBS
Clone No.1F3G11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CCNB1, 1F3G11, CCNB, Cyclin B, G2/mitotic specific cyclin B1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67686-1-PBS targets cyclin B1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29444

Product name: Recombinant human CCNB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-140 aa of BC006510

Sequence: MALRVTRNSKINAENKAKINMAGAKRVPTAPAATSKPGLRPRTALGDIGNKVSEQLQAKMPMKKEAKPSATGKVIDKKLPKPLEKVPMLVPVPVSEPVPEPEPEPEPEPVKEEKLSPEPILVDTASPSPMETSGCAPAEE

相同性解析による交差性が予測される生物種
Full Name cyclin B1
Calculated molecular weight 48 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC006510
Gene Symbol Cyclin B1
Gene ID (NCBI) 891
RRIDAB_2882879
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP14635
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.