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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using cIAP1 Monoclonal antibody (66626-1-Ig)

    WB analysis using 66626-1-Ig (same clone as 66626-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66626-1-Ig (cIAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using cIAP1 Monoclonal antibody (66626-1-Ig)

    WB analysis using 66626-1-Ig (same clone as 66626-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66626-1-Ig (cIAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human spleen using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human spleen using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human lung cancer using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human lymphoma using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human lymphoma using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human tonsillitis using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using cIAP1 Monoclonal antibody (66626-1-Ig)

    IHC staining of human tonsillitis using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66626-1-Ig (cIAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using cIAP1 Monoclonal antibody (66626-1-Ig)

    IF Staining of HepG2 using 66626-1-Ig (same clone as 66626-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66626-1-Ig (cIAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66626-1-PBS in a different storage buffer formulation.

cIAP1 Monoclonal antibody, PBS Only

cIAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66626-1-PBS
Clone No.1H3F1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

API1, BIRC2, MIHB, RNF48, 1H3F1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66626-1-PBS targets cIAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21398

Product name: Recombinant human cIAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 95-196 aa of BC016174

Sequence: LGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPL

相同性解析による交差性が予測される生物種
Full Name baculoviral IAP repeat-containing 2
Calculated molecular weight 618 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC016174
Gene Symbol cIAP1
Gene ID (NCBI) 329
RRIDAB_2881986
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ13490
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

BIRC2 (also known as cIAP1) is a member of the inhibitor of apoptosis protein (IAP) family. The inhibitor of apoptosis (IAP) proteins are a family of anti-apoptotic regulators found in viruses and metazoans. BIRC2 is a nuclear shuttling protein, whose subcellular localization is mediated by the CRM1-dependent nuclear export pathway (PMID: 15265700). The protein is regulated transcriptionally and can be inhibited by mitochondrial proteins released in the cytoplasm upon apoptotic stimuli (PMID: 15187025). BIRC2 is also believed to be a critical regulator of vascular integrity and endothelial cell survival, thereby providing an additional target pathway for the control of angiogenesis and blood vessel homeostasis during embryogenesis, regeneration and tumorigenesis (PMID: 17934460).

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