Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)ChIP-qPCRAffinity

WB analysis of A549 using 80845-1-RR (same clone as 80845-1-PBS)
WB result of MYC antibody (80845-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYC transfected A549 cells. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
WB analysis using 80845-1-RR (same clone as 80845-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 80845-1-RR (c-MYC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
WB analysis using 80845-1-RR (same clone as 80845-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 80845-1-RR (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 80845-1-RR (same clone as 80845-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 80845-1-RR (same clone as 80845-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
IHC staining of mouse embryo using 80845-1-RR (same clone as 80845-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 80845-1-RR (same clone as 80845-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 80845-1-RR (same clone as 80845-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 80845-1-RR (same clone as 80845-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 80845-1-RR (same clone as 80845-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 80845-1-RR (same clone as 80845-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Recombinant antibody (80845-1-RR, Clone:2B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
×
1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Recombinant antibody (80845-1-RR, Clone:2B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
ChIP experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of c-MYC (80845-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
×
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of c-MYC (80845-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80845-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 80845-1-RR
Biolayer interferometry (BLl) kinetic assays of 80845-1-RR against Human c-MYC were performed. The affinity constant is 2.03 nM.

Featured Product
KD/KO Validated

c-MYC Recombinant monoclonal antibody, PBS Only

c-MYC Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Cat No. 80845-1-PBS

Clone No.2B17

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR

Synonyms

MYC, 2B17, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80845-1-PBS targets c-MYC in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1263 Product name: Recombinant human MYC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 16-454 aa of BC000141 Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA 相同性解析による交差性が予測される生物種
Full Name	v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight	49 kDa
Observed molecular weight	50-65 kDa
GenBank accession number	BC000141
Gene Symbol	MYC
Gene ID (NCBI)	4609
RRID	AB_2918915
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P01106
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

MYC contains a basic helix-loop-helix (bHLH) domain. This protein is a multifunctional nuclear phosphoprotein, which plays a role in cell cycle process, apoptosis and cell transformation. As a transcription factor, it regulates the transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds to DNA in a nonspecific way, but also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutation, overexpression, rearrangement and translocation of this gene are related to many hematopoietic tumors, leukemia and lymphoma (including Burkitt lymphoma). The antibody is a rabbit polyclonal antibody against human MYC recombinant protein. The 50kDa band recognized by antibody is the natural form of MYC, while other bands between 60 and 70 kDa are the phosphorylated form of MYC (PMID: 12189186). The latest research reveals a new key mechanism of MYC protein, which is independent of its classical transcriptional regulation function. It was found that under the stress of transcription, MYC was polymerized through its specific RNA binding domain (RBRIII), and exocrine was targeted at the nuclear R loop and double-stranded RNA. This process can effectively eliminate the immunogenic RNA-DNA hybrid from R-loop, thus preventing it from activating TLR3-TBK1 natural immune signaling pathway. This mechanism is very important for MYC to maintain tumor growth and achieve immune escape in vivo. (PMID: 41576951)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 80845-1-RR (same clone as 80845-1-PBS)

WB analysis using 80845-1-RR (same clone as 80845-1-PBS)

WB analysis using 80845-1-RR (same clone as 80845-1-PBS)

IHC staining of human colon using 80845-1-RR (same clone as 80845-1-PBS)

IHC staining of human colon cancer using 80845-1-RR (same clone as 80845-1-PBS)

IHC staining of mouse embryo using 80845-1-RR (same clone as 80845-1-PBS)

IHC staining of human tonsillitis using 80845-1-RR (same clone as 80845-1-PBS)

IHC staining of human tonsillitis using 80845-1-RR (same clone as 80845-1-PBS)

IF Staining of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

IF Staining of HEK-293 using 80845-1-RR (same clone as 80845-1-PBS)

IF Staining of U2OS using 80845-1-RR (same clone as 80845-1-PBS)

FC experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

ChIP experiment of HeLa using 80845-1-RR (same clone as 80845-1-PBS)

Affinity and Kinetic Characterization of 80845-1-RR