Validation Data Gallery

  • Co-IP of BAX and BAK by anti-rabbit IgG VHH agarose (rIGma) using a BAX polyclonal IgG (Proteintech: 50599-2-Ig). As control an IgG isotype control antibody (Proteintech: 98136-1-RR) was used (BISO). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of input (IN) and flow through (FT) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis BAX was detected using a polyclonal rabbit IgG (Proteintech: 50599-2-Ig) (1:2000) labeled using FlexAble HRP (Proteintech: KFA005). The presence of BAK co-precipitated with BAX was confirmed using a polyclonal rabbit IgG (Proteintech: 29552-1-AP) (1:2000). While rIGa allows for clean detection of both proteins, Protein A magnetic beads show additional bands, probably due to leaching of Protein A from the beads into the IP fractions, which can lead to binding of the detection antibody and unspecific signals.

    Co-IP of BAX and BAK by anti-rabbit IgG VHH agarose (rIGma) using a BAX polyclonal IgG (Proteintech: 50599-2-Ig). As control an IgG isotype control antibody (Proteintech: 98136-1-RR) was used (BISO). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of input (IN) and flow through (FT) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis BAX was detected using a polyclonal rabbit IgG (Proteintech: 50599-2-Ig) (1:2000) labeled using FlexAble HRP (Proteintech: KFA005). The presence of BAK co-precipitated with BAX was confirmed using a polyclonal rabbit IgG (Proteintech: 29552-1-AP) (1:2000). While rIGa allows for clean detection of both proteins, Protein A magnetic beads show additional bands, probably due to leaching of Protein A from the beads into the IP fractions, which can lead to binding of the detection antibody and unspecific signals.

  • Co-IP of MCM complex via pulldown of MCM6 using 5 µg of polyclonal rabbit anti-MCM6 antibody (PTG: 13347-2-AP) and anti-rabbit IgG magnetic VHH agarose (rIGma). All subunits of the 600 kDa hetero-hexameric complex are successfully precipitated, as shown in a Coomassie blue stained SDS-gel and by Western blot analysis using subunit specific antibodies. Apparent molecular weights are provided. For input (IN) and flowthrough (FT) fractions, 1% was loaded, respectively. For bound (B) fraction and bound fraction of isotype control antibody (BISO PTG: 98136-1-RR), 20% was loaded. Product codes for Proteintech antibodies are provided in parentheses.

    Co-IP of MCM complex via pulldown of MCM6 using 5 µg of polyclonal rabbit anti-MCM6 antibody (PTG: 13347-2-AP) and anti-rabbit IgG magnetic VHH agarose (rIGma). All subunits of the 600 kDa hetero-hexameric complex are successfully precipitated, as shown in a Coomassie blue stained SDS-gel and by Western blot analysis using subunit specific antibodies. Apparent molecular weights are provided. For input (IN) and flowthrough (FT) fractions, 1% was loaded, respectively. For bound (B) fraction and bound fraction of isotype control antibody (BISO PTG: 98136-1-RR), 20% was loaded. Product codes for Proteintech antibodies are provided in parentheses.

ChromoTek anti-Rabbit IgG Magnetic VHH Agarose for Immunoprecipitation

anti-Rabbit IgG IP Beads is an affinity resin for IP of Rabbit IgG. It consists of rabbit specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.
Cat No. rIGma

Reactivity

Rabbit IgG

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Magnetic Agarose

Rabbit IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody

Formats:  Magnetic Agarose
Size/Concentration: 

-/ -


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Product Information

anti-Rabbit IgG IP Beads is an affinity resin for IP of Rabbit IgG. It consists of rabbit specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.

DescriptionReady to use VHH coupled Agarose Beads for Immunoprecipitation of Rabbit IgG. Add bulletpoints from below here:

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• Stable in harsh buffer conditions

• Suitable for downstream mass spec analysis

• High Specificity

• Low background

ApplicationsIP, Co-IP
Binding capacity/
ConjugateMagnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
Elution bufferSDS Sample Buffer
Wash buffer compatibility/
TypeNanobody
ClassRecombinant - Animal free production
HostAlpaca
Affinity (KD)/
ImmunogenRabbit IgG
RRIDAB_3697091
Storage Buffer20% Ethanol
Storage Condition+4°C / do not freeze!
ShippingAmbient Temp
Size1 ml; 2 ml; 5 ml