Validation Data Gallery

  • Co-IP of MCM complex via pulldown of MCM6 using 5 µg of monoclonal anti-MCM6 IgG1 (Proteintech: 67989-1-Ig) and anti-mouse IgG1 VHH agarose (mIG1a). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 1x10^7 cells. All subunits of the 600 kDa hetero-hexameric complex are successfully precipitated, as shown in a Coomassie blue stained SDS-gel and by Western blot analysis using subunit specific antibodies. Apparent molecular weights are provided. For input (IN) and flowthrough (FT) fractions, 1% was loaded, respectively. For bound (B) fraction and bound fraction of isotype control antibody (BISO Proteintech: 66360-1-Ig), 25% was loaded. Product codes for Proteintech-antibodies are provided in parentheses. Compared to Protein A agarose, mIG1a shows a superior precipitation of the MCM complex.

    Co-IP of MCM complex via pulldown of MCM6 using 5 µg of monoclonal anti-MCM6 IgG1 (Proteintech: 67989-1-Ig) and anti-mouse IgG1 VHH agarose (mIG1a). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 1x10^7 cells. All subunits of the 600 kDa hetero-hexameric complex are successfully precipitated, as shown in a Coomassie blue stained SDS-gel and by Western blot analysis using subunit specific antibodies. Apparent molecular weights are provided. For input (IN) and flowthrough (FT) fractions, 1% was loaded, respectively. For bound (B) fraction and bound fraction of isotype control antibody (BISO Proteintech: 66360-1-Ig), 25% was loaded. Product codes for Proteintech-antibodies are provided in parentheses. Compared to Protein A agarose, mIG1a shows a superior precipitation of the MCM complex.

  • IP of PCNA by anti-mouse IgG1 VHH agarose (mIG1a) using a monoclonal PCNA antibody (Proteintech: 60097-1-Ig). As control, a IgG1 isotype control antibody (Proteintech: 66360-1-Ig) was used (BISO). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of input (IN) and flow through (FT) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis PCNA was detected using a polyclonal rabbit IgG (Proteintech: 10205-2-AP) (1:5000) and an HRP-labeled goat anti-rabbit secondary (Proteintech: RGAR001). Compared to Protein A agarose, mIG1a shows a superior precipitation of PCNA.

    IP of PCNA by anti-mouse IgG1 VHH agarose (mIG1a) using a monoclonal PCNA antibody (Proteintech: 60097-1-Ig). As control, a IgG1 isotype control antibody (Proteintech: 66360-1-Ig) was used (BISO). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 0.5x10^7 cells. 1% of input (IN) and flow through (FT) and 25% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis PCNA was detected using a polyclonal rabbit IgG (Proteintech: 10205-2-AP) (1:5000) and an HRP-labeled goat anti-rabbit secondary (Proteintech: RGAR001). Compared to Protein A agarose, mIG1a shows a superior precipitation of PCNA.

ChromoTek anti-Mouse IgG1 VHH Agarose for Immunoprecipitation

anti-Mouse IgG1 IP Beads is an affinity resin for IP of Mouse IgG1. It consists of rabbit specific VHHs (Nanobodies) coupled to Agarose beads.
Cat No. mIG1a

Reactivity

Mouse IgG1

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Mouse IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody

Formats:  Agarose
Size/Concentration: 

-/ -


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Product Information

anti-Mouse IgG1 IP Beads is an affinity resin for IP of Mouse IgG1. It consists of rabbit specific VHHs (Nanobodies) coupled to Agarose beads.

Description

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• Stable in harsh buffer conditions

• Suitable for downstream mass spec analysis

• High Specificity

• Low background

ApplicationsIP, Co-IP
Binding capacity/
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS Sample Buffer
Wash buffer compatibility/
TypeNanobody
ClassRecombinant - Animal free production
HostAlpaca
Affinity (KD)/
ImmunogenMouse IgG1
RRIDAB_3697092
Storage Buffer20% Ethanol
Storage Condition+4°C / do not freeze!
ShippingAmbient Temp
Size1 ml; 2 ml; 5 ml