Validation Data Gallery
Product Information
Bivalent anti-HA-Tag VHH (Nanobody) that is conjugated to fluorescent dyes for immunofluorescence, microscopy, and immunoblotting of HA-tagged proteins.
| Description | HA-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to its small size, HA-Label is optimal for effective labeling with minimal epitope-label displacement for imaging applications, escpecially super-resolution microscopy. The small size further leads to superior accessibility and labelling of epitopes in crowded cellular/organelle environments. High affinity due to strong avidity effect from bivalent format of the HA-Label. |
| Applications | IF, WB |
| Reactivity | HA Tag |
| Conjugate | CoraLite® Plus 647 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity | 1:1 affinity 6 nM C-term. HA, 180 nM N-term. HA. But higher in IF and WB owing to its bivalent format and hence avidity effects |
| Molecular Weight | 28.5 kDa |
| Form | Liquid |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| RRID | AB_3665419 |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide |
| Storage condition | -20°C |
| Shipping | blue ice |