Use Able AI chat for product recommendations

Validation Data Gallery

  • <b>Human PBMCs stained with anti-CD33 recombinant VHH  in Flow Cytometry</b><br/>
1x10^6 human PBMCs were surface stained in 100 µL with 1 µg anti-CD33 recombinant VHH, CoraLite® Plus 555 (MG-1230CL555, Clone: CTK0243) or unstained. Fc-receptors were blocked. Cells were not fixed. Monocytes were gated.

    Human PBMCs stained with anti-CD33 recombinant VHH  in Flow Cytometry
    1x10^6 human PBMCs were surface stained in 100 µL with 1 µg anti-CD33 recombinant VHH, CoraLite® Plus 555 (MG-1230CL555, Clone: CTK0243) or unstained. Fc-receptors were blocked. Cells were not fixed. Monocytes were gated.

  • <b>Human PBMCs stained with anti-CD33 recombinant VHH  in Flow Cytometry</b><br/>
1x10^6 human PBMCs were surface stained in 100 µL with 1 µg anti-CD33 recombinant VHH, CoraLite® Plus 555 (MG-1230CL555, Clone: CTK0243) (red) or unstained (blue). Fc-receptors were blocked. Cells were not fixed. Monocytes were gated.

    Human PBMCs stained with anti-CD33 recombinant VHH  in Flow Cytometry
    1x10^6 human PBMCs were surface stained in 100 µL with 1 µg anti-CD33 recombinant VHH, CoraLite® Plus 555 (MG-1230CL555, Clone: CTK0243) (red) or unstained (blue). Fc-receptors were blocked. Cells were not fixed. Monocytes were gated.

  • <b>Human PBMCs co-stained with anti-CD33 recombinant VHH and anti-CD14  26IC  in Flow Cytometry</b><br/>
1x10^6 human PBMCs were surface co-stained in 100 µL with 1 µg anti-CD33 recombinant VHH, CoraLite® Plus 555 (MG-1230CL555, Clone: CTK0243), or 1 µg recombinant Alpaca VHH Isotype Control (Clone: CTK0201), and 5 µL CoraLite® Plus 647 Anti-Human CD14 (CL647-65616). Fc-receptors were blocked. Cells were not fixed.

    Human PBMCs co-stained with anti-CD33 recombinant VHH and anti-CD14  26IC  in Flow Cytometry
    1x10^6 human PBMCs were surface co-stained in 100 µL with 1 µg anti-CD33 recombinant VHH, CoraLite® Plus 555 (MG-1230CL555, Clone: CTK0243), or 1 µg recombinant Alpaca VHH Isotype Control (Clone: CTK0201), and 5 µL CoraLite® Plus 647 Anti-Human CD14 (CL647-65616). Fc-receptors were blocked. Cells were not fixed.

  • <b>Human PBMCs co-stained with anti-CD33 recombinant VHH and anti-CD14  26IC  in Flow Cytometry</b><br/>
1x10^6 human PBMCs were surface co-stained in 100 µL with 1 µg anti-CD33 recombinant VHH, CoraLite® Plus 555 (MG-1230CL555, Clone: CTK0243) (red), or 1 µg recombinant Alpaca VHH Isotype Control (Clone: CTK0201, blue) and 5 µL Anti-Human CD1, CoraLite® Plus 647 (CL647-65616). Fc-receptors were blocked. Cells were not fixed.

    Human PBMCs co-stained with anti-CD33 recombinant VHH and anti-CD14  26IC  in Flow Cytometry
    1x10^6 human PBMCs were surface co-stained in 100 µL with 1 µg anti-CD33 recombinant VHH, CoraLite® Plus 555 (MG-1230CL555, Clone: CTK0243) (red), or 1 µg recombinant Alpaca VHH Isotype Control (Clone: CTK0201, blue) and 5 µL Anti-Human CD1, CoraLite® Plus 647 (CL647-65616). Fc-receptors were blocked. Cells were not fixed.

ChromoTek anti-CD33 recombinant VHH, CoraLite ® Plus 555

The small size of VHHs (~15 kDa) enables access to epitopes that may be sterically inaccessible to full size IgGs. Their reduced steric hindrance also facilitates simultaneous staining of multiple targets, making them particularly useful for applications like Nano-Flow. MG-1230CL555 is a recombinant CoraLite ® Plus 555 conjugated anti CD33 Nanobody (VHH) that targets CD33 (human).
Cat No. MG-1230CL555

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 555

Siglec-3 VHH, p67 VHH, gp67 VHH

Size: 

-/ -

Concentration: 0.5 mg/ml


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Product Information

The small size of VHHs (~15 kDa) enables access to epitopes that may be sterically inaccessible to full size IgGs. Their reduced steric hindrance also facilitates simultaneous staining of multiple targets, making them particularly useful for applications like Nano-Flow. MG-1230CL555 is a recombinant CoraLite ® Plus 555 conjugated anti CD33 Nanobody (VHH) that targets CD33 (human).

ApplicationsFC
ReactivityHuman
ConjugateCoraLite® Plus 555
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity270 nM
CloneCTK0243
Immunogen18-259 aa
Molecular Weight15.7 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
RRID/
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
Shippingblue ice
Sizes150 µl
Amount per test (Flow Cytometry)2 µl per test
Total number of tests75 tests
BackgroundCD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
||
New chat

Able™

正在加载,请稍候...