Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
| Description | Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below. |
| Applications | IF, WB, FC |
| Host | Alpaca |
| Species Reactivity | Mouse IgG1 Fc-fragment No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3 |
| Target/Specificity | This Nanobody mixture recognizes the Fc fragment of mouse IgG1. |
| Physical State | Liquid |
| Conjugate | Alexa Fluor® 488 |
| Suggested Dilution | Immunofluorescence 1:500 Super-resolution microscopy 1:500 Western blot 1:1,000 |
| Purification Method | Recombinant expression, affinity purification IMAC |
| Type | Mixture of 2 monoclonal Nanobodies; Secondary Nanobody |
| Class | Recombinant |
| Format | Alpaca single domain antibodies, monovalent |
| Cross-reactivity | No cross-reactivity to rabbit, rat, sheep, goat, guinea pig, human, and macaque serum |
| Immunogen | Purified mouse IgG1 |
| Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
| Clones | VHH0302, VHH0305 |
| RRID | AB_2827579 |
| Affinity (KD) | CTK0103: KD =0.13 nM, CTK0104: KD = 0.63nM |
| Excitation/ Emission | Excitation max: 490 nm, Emission max: 525 nm |
| Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
| Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
| Size | 10 µL; 100 µL |
| Note | This product is for research use only, not for diagnostic or therapeutic use |
Documentation
| SDS |
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| sms1AF488-1_SDS_Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (EN) |
| Datasheet |
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| Protocol Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor 488 (PDF) |
| Application note |
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| Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
| One-step incubation with Nano-Secondaries (PDF) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Biomater Res Bioactive self-healing hydrogel based on tannic acid modified gold nano-crosslinker as an injectable brain implant for treating Parkinson's disease | |
Int J Biol Macromol zwitterionic Pluronic analog-coated PLGA nanoparticles for oral insulin delivery | |
iScience Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress | |
Pharmaceuticals (Basel) GRK2 Mediates Macrophage Polarization by Regulating EP4-cAMP-pCREB Signaling in Ulcerative Colitis and the Therapeutic Effect of Paroxetine on Mice with DSS-Induced Colitis | |
Biol Trace Elem Res ERK/PKM2 Is Mediated in the Warburg Effect and Cell Proliferation in Arsenic-Induced Human L-02 Hepatocytes | |
Mol Neurobiol Transcription Factor BCL11A Regulates Schwann Cell Behavior During Peripheral Nerve Regeneration |