Validation Data Gallery
Tested Applications
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-80008-2 targets a-SMA specific in IF-P applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated molecular weight | 42 kDa |
| GenBank accession number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha smooth muscle actin (α-SMA or ACTA2) is an actin isoform predominantly expressed in smooth muscle cells and is the most widely recognized marker for activated myofibroblasts. In healthy adult tissues, its expression is largely restricted to vascular and visceral smooth muscle. However, during tissue injury, fibrosis, or wound healing, fibroblasts differentiate into myofibroblasts with significant upregulation of α-SMA. Therefore, α-SMA serves as a critical immunohistochemical marker for detecting myofibroblast activation and studying pathological processes such as organ fibrosis (e.g., liver, kidney, lung) and cancer stroma remodeling.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 a-SMA specific antibody CL647-80008-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |