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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZYG11A Polyclonal antibody (30597-1-AP)

    WB analysis of HeLa using 30597-1-AP (same clone as 30597-1-PBS)

    WB result of ZYG11A antibody (30597-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZYG11A transfected HeLa cells. This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZYG11A Polyclonal antibody (30597-1-AP)

    WB analysis using 30597-1-AP (same clone as 30597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30597-1-AP (ZYG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZYG11A Polyclonal antibody (30597-1-AP)

    WB analysis using 30597-1-AP (same clone as 30597-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30597-1-AP (ZYG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using ZYG11A Polyclonal antibody (30597-1-AP)

    IF Staining of K-562 using 30597-1-AP (same clone as 30597-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ZYG11A antibody (30597-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30597-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZYG11A Polyclonal antibody, PBS Only

ZYG11A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30597-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Protein zyg 11 homolog A, ZYG11

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30597-1-PBS targets ZYG11A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30166

Product name: Recombinant human ZYG11A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 628-696 aa of NM_001004339

Sequence: TSDRQLWISRDFQRRTLLQDLHATIQNWPSSSCKMTALVTYRSFKTFFPLLGNFSQPEVQLWALWAMYH

相同性解析による交差性が予測される生物種
Full Name zyg-11 homolog A (C. elegans)
Calculated molecular weight 86 kDa
Observed molecular weight48 kDa
GenBank accession numberNM_001004339
Gene Symbol ZYG11A
Gene ID (NCBI) 440590
RRIDAB_3742356
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6WRX3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZYG11A is a member of the Zyg11 protein family that functions as a target recruitment subunit of an E3 ubiquitin ligase complex playing a central role in the degradation of a regulatory protein involved in cell cycle regulation. ZYG11A is over-expressed in NSCLC tumor tissues and correlates with more aggressive clinical characteristics. Also ZYG11A gene constitutes a novel target for IGF1 action in endometrial cancer cells (PMID: 26771237, 31320996). This antibody is specific to isoform 2 of ZYG11A with a predicted molecular mass of 48 kDa.

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