Validation Data Gallery
Tested Applications
| Positive WB detected in | NCI-H1299 cells, HEK-293T cells, HeLa cells |
| Positive IF/ICC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30597-1-AP targets ZYG11A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30166 Product name: Recombinant human ZYG11A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 628-696 aa of NM_001004339 Sequence: TSDRQLWISRDFQRRTLLQDLHATIQNWPSSSCKMTALVTYRSFKTFFPLLGNFSQPEVQLWALWAMYH 相同性解析による交差性が予測される生物種 |
| Full Name | zyg-11 homolog A (C. elegans) |
| Calculated molecular weight | 86 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | NM_001004339 |
| Gene Symbol | ZYG11A |
| Gene ID (NCBI) | 440590 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6WRX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZYG11A is a member of the Zyg11 protein family that functions as a target recruitment subunit of an E3 ubiquitin ligase complex playing a central role in the degradation of a regulatory protein involved in cell cycle regulation. ZYG11A is over-expressed in NSCLC tumor tissues and correlates with more aggressive clinical characteristics. Also ZYG11A gene constitutes a novel target for IGF1 action in endometrial cancer cells (PMID: 26771237, 31320996). This antibody is specific to isoform 2 of ZYG11A with a predicted molecular mass of 48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZYG11A antibody 30597-1-AP | Download protocol |
| WB protocol for ZYG11A antibody 30597-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |