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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using ZRANB2 Polyclonal antibody (24816-1-AP)

    WB analysis of NIH/3T3 using 24816-1-AP (same clone as 24816-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24816-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ZRANB2 Polyclonal antibody (24816-1-AP)

    WB analysis using 24816-1-AP (same clone as 24816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24816-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ZRANB2 Polyclonal antibody (24816-1-AP)

    WB analysis of Jurkat using 24816-1-AP (same clone as 24816-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24816-1-AP (ZRANB2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24816-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZRANB2 Polyclonal antibody, PBS Only

ZRANB2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 24816-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24816-1-PBS targets ZRANB2 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20487

Product name: Recombinant human ZRANB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-241 aa of BC039814

Sequence: MSTKNFRVSDGDWICPDKKCGNVNFARRTSCNRCGREKTTEAKMMKAGGTEIGKTLAEKSRGLFSANDWQCKTCSNVNWARRSECNMCNTPKYAKLEERTGYGGGFNERENVEYIEREESDGEYDEFGRKKKKYRGKAVGPASILKEVEDKESEGEEEDEDEDLSKYKLDEDEDEDDADLSKYNLDASEEEDSNKKKSNRRSRSKSGSSHSRSSSRSSSPSSSRSRSRSRSRSSSSSQSRS

相同性解析による交差性が予測される生物種
Full Name zinc finger, RAN-binding domain containing 2
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight37 kDa, 48 kDa
GenBank accession numberBC039814
Gene Symbol ZRANB2
Gene ID (NCBI) 9406
RRIDAB_2879740
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95218
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZRANB2, also named as ZIS and ZNF265, is a splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. It may interfere with constitutive 5'-splice site selection. The calculated MW of ZRANB2 is 330aa, 37 kDa or 320aa, 36 kDa. We always got the ~48 kDa in our detection. The immunogen of this antibody is 1-241aa. No other protein can be recognized by this antibody from the sequence BLAST results.

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