Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
24816-1-AP targets ZRANB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZRANB2 fusion protein Ag20487 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger, RAN-binding domain containing 2 |
| Calculated molecular weight | 330 aa, 37 kDa |
| Observed molecular weight | 37 kDa, 48 kDa |
| GenBank accession number | BC039814 |
| Gene Symbol | ZRANB2 |
| Gene ID (NCBI) | 9406 |
| RRID | AB_2879740 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95218 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZRANB2, also named as ZIS and ZNF265, is a splice factor required for alternative splicing of TRA2B/SFRS10 transcripts. It may interfere with constitutive 5'-splice site selection. The calculated MW of ZRANB2 is 330aa, 37 kDa or 320aa, 36 kDa. We always got the ~48 kDa in our detection. The immunogen of this antibody is 1-241aa. No other protein can be recognized by this antibody from the sequence BLAST results.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZRANB2 antibody 24816-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res ZRANB2 and SYF2-mediated splicing programs converging on ECT2 are involved in breast cancer cell resistance to doxorubicin. | ||
Aging (Albany NY) LncRNA FAM181A-AS1 promotes gliomagenesis by sponging miR-129-5p and upregulating ZRANB2. | ||
J Exp Clin Cancer Res ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma.
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