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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of SiHa cells using ZNF750 Polyclonal antibody (21752-1-AP)

    IP experiment of SiHa using 21752-1-AP (same clone as 21752-1-PBS)

    IP result of anti-ZNF750 (IP:21752-1-AP, 4ug; Detection:21752-1-AP 1:500) with SiHa cells lysate 1520 ug. This data was developed using the same antibody clone with 21752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using ZNF750 Polyclonal antibody (21752-1-AP)

    IHC staining of human oesophagus cancer using 21752-1-AP (same clone as 21752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21752-1-AP (ZNF750 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using ZNF750 Polyclonal antibody (21752-1-AP)

    IHC staining of human oesophagus cancer using 21752-1-AP (same clone as 21752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 21752-1-AP (ZNF750 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using ZNF750 Polyclonal antibody (21752-1-AP)

    IHC staining of human skin using 21752-1-AP (same clone as 21752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21752-1-AP (ZNF750 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using ZNF750 Polyclonal antibody (21752-1-AP)

    IHC staining of human skin using 21752-1-AP (same clone as 21752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21752-1-AP (ZNF750 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using ZNF750 Polyclonal antibody (21752-1-AP)

    IHC staining of human skin cancer using 21752-1-AP (same clone as 21752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21752-1-AP (ZNF750 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21752-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using ZNF750 Polyclonal antibody (21752-1-AP)

    IHC staining of human skin cancer using 21752-1-AP (same clone as 21752-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21752-1-AP (ZNF750 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21752-1-PBS in a different storage buffer formulation.

ZNF750 Polyclonal antibody, PBS Only

ZNF750 Polyclonal Antibody for IHC, IP, ELISA
Cat No. 21752-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, ELISA

Synonyms

Protein ZNF750, Zfp750, Zinc finger protein 750

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21752-1-PBS targets ZNF750 in IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16116

Product name: Recombinant human ZNF750 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 374-723 aa of BC109037

Sequence: HVEFESPIPEAKDSSKAGQRDTEGSKMSPRAGSAATGSPGRPSPTDFMQTSQTCEGLYDLSNKAASSALGRLYPPEQSLTAFRPVKKSTECLPAQAAETTAESPVSLNVVNGDPPAPTGSASLVSEAAPSSPDDSSGMGPLNLSKKSEINLAATHEPTYQGSPQAETASFSELQDLPLNLSVKDPCNTQAPRPAFPGRPRAAEPAAAVPQKTGTEGSEDGPSHPETKPGSLDGDGAPPTGPGEEAPDACAVDSSEEQKQTAAVALCQLAAYSPRNIRVGDGDAAAPEPACRQDTPTLSSMESQEAQCDLRPKGQKRTSLRDAGKSQQGAKKAKLQDTARVFTLRRRARVS

相同性解析による交差性が予測される生物種
Full Name zinc finger protein 750
Calculated molecular weight 723 aa, 77 kDa
Observed molecular weight 70-77 kDa
GenBank accession numberBC109037
Gene Symbol ZNF750
Gene ID (NCBI) 79755
RRIDAB_10863125
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ32MQ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ZNF750 is a novel nuclear protein highly expressed in differentiated skin keratinocytes. Mutation of ZNF750 is linked with familial psoriasis. Recently ZNF was found to be significantly mutated in esophageal squamous cell carcinoma (ESCC). Expression of ZNF750 is decreased in ESCC compared to the normal tissue.

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