Validation Data Gallery
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Tested Applications
| Positive IP detected in | SiHa cells |
| Positive IHC detected in | human oesophagus cancer tissue, human skin tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
21752-1-AP targets ZNF750 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZNF750 fusion protein Ag16116 相同性解析による交差性が予測される生物種 |
| Full Name | zinc finger protein 750 |
| Calculated molecular weight | 723 aa, 77 kDa |
| Observed molecular weight | 70-77 kDa |
| GenBank accession number | BC109037 |
| Gene Symbol | ZNF750 |
| Gene ID (NCBI) | 79755 |
| RRID | AB_10863125 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q32MQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ZNF750 is a novel nuclear protein highly expressed in differentiated skin keratinocytes. Mutation of ZNF750 is linked with familial psoriasis. Recently ZNF was found to be significantly mutated in esophageal squamous cell carcinoma (ESCC). Expression of ZNF750 is decreased in ESCC compared to the normal tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZNF750 antibody 21752-1-AP | Download protocol |
| IP protocol for ZNF750 antibody 21752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Cancer Identification of calmodulin-like protein 5 as tumor-suppressor gene silenced during early stage of carcinogenesis in squamous cell carcinoma of uterine cervix. | ||
Oncol Lett Differentiation-related zinc finger protein 750 suppresses cell growth in esophageal squamous cell carcinoma. | ||
Cell Death Discov TRIM29 hypermethylation drives esophageal cancer progression via suppression of ZNF750 | ||
Cell Death Dis GPR37-enhanced ubiquitination of ATP1A1 inhibits tumor progression and radiation resistance in esophageal squamous cell carcinoma |