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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZMYND10 Recombinant antibody (86430-1-RR)

    WB analysis using 86430-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86430-1-RR (ZMYND10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZMYND10 Recombinant antibody (86430-1-RR)

    WB analysis using 86430-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86430-1-RR (ZMYND10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86430-1-RR

    Affinity and Kinetic Characterization of 86430-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86430-1-RR against Human ZMYND10 were performed. The affinity constant is below 1 pM.

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ZMYND10 Recombinant antibody

ZMYND10 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86430-1-RR
Clone No.251276H4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BLU, LUCA12.4, Protein BLu, Zinc finger MYND domain-containing protein 10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86430-1-RR targets ZMYND10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZMYND10 fusion protein Ag5808 相同性解析による交差性が予測される生物種
Full Name zinc finger, MYND-type containing 10
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC033732
Gene Symbol ZMYND10
Gene ID (NCBI) 51364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75800
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZMYND10 (Zinc finger MYND domain-containing protein 10), also known as BLU, encodes a 50-kDa protein containing an MYND-type zinc finger DNA-binding domain in the C-terminus that is commonly found in transcription repressors. ZMYND10 is highly enriched in ciliated cells compared with that in nonciliated cells and is expressed in motile ciliated tissues in mice (PMID: 23891471). ZMYND10 is a tumor suppressor that can induce apoptosis, arrest cell cycle, and inhibit proliferation and angiogenesis in different tumors (PMID: 29601588, 31801619).

Protocols

Product Specific Protocols
WB protocol for ZMYND10 antibody 86430-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols